Varying the amount of light alters the image contrast. G. Mirror/ Light source: brightens up the view so the object can be seen more clearly H- Course focus knob: brings the object into the focal plane of the objective lens. – Fine focus knob: makes fine adjustments to focus the image. J- Arm: a curved portion that holds all of the optical parts at a fixed distance and aligns them K- Stage Clips: holds the specimen on the stage. When looking at a magnified image, even moving the specimen slightly can move parts of the image out of view.
Base: supports the weight of all of the microscope parts. Define the following microscopy terms: ; Focus: Focus is a means of moving the specimen closer or further away from the objective lens to be able to see a sharp image, ; Resolution: Resolution is the amount of detail you can see in an image. ; Contrast: Contrast is defined as the difference in light intensity between the image and the adjacent background relative to the overall background intensity. The purpose Of immersion Oil is to prevent the refraction Of light.
Immersion Oil works by immersing both the objective lens and the specimen in transparent oil, now as immersion Oil, therefore increasing the amount Of light that can pass through the objective lens. Draw pictures of bacteria page 65 examples The Cisco look like small round balls. The streptococci remind me of the little Timmy silver balls that magnetite together and form a chain. The staphylococci remind me of the same balls only clustered together, much like a bowl of dip and dot ice cream. Lastly there is bacillus, which look much like rice that are either single grains, chains, or clusters.
Exercise 2: observing Bacteria cultures in yogurt questions I. Describe your observations of the fresh yogurt slide. From observation to fresh yogurt slide was able to see some rod shaped and some round or spherical bacteria. 2. Were there observable differences between your fresh yogurt slide and the prepared yogurt slide? If so, explain. There were small observable differences in the fresh and prepared yogurt slides. The prepared slide seemed to be a little clearer as tar as being able to identity the different bacterial shapes than the fresh yogurt was. Surly this has to do with gestational time.
In my opinion there were more bacterial cells on the prepared slide than there was the fresh slide. This could also have to do With the type of yogurt used. 3. Describe the four main bacterial shapes. The four main bacterial shapes are spheres, rods, commas and spirals. Spherical bacteria are called Cisco and look like small little balls. Rod shaped bacteria is called bacillus and looks like a macaroni noodle. The comma bacteria is called a brio and looks just as it sounds, like a comma. Lastly there is the spiral, which is really called the spiritual. 4. What are the common arrangements of bacteria? Diploid – means paired.
Streets – means linked in chains. Soothsay – means grouped in clusters, . Were you able to identity specific bacterial morphologies on either yogurt slide? If 50, which types? Was not able to identify specific bacterial morphologies on my slides. Imagine there are some that I should be able to identify but hopefully as I become more familiar with the microscope and become a better user I will be able to identify specific bacterial morphologies. Exercise 3: Preparing and observing a Blood Slide A. Describe the cells you were able to see in the blood smear. The cells that I avgas able to see in the blood smear were red blood cells.
There were thousands of them. Deiced in my slide they appeared darker around the edges of the smear. Was able to see a few white blood cells that looks to me like interruptions, and sinkhole’s. I’m sure there were hundreds Of many different types Of cells but those are the ones I was able to identify. B. Are the cells you observed in your blood smear different than the bacterial cells you have observed? Why or Why not? The yogurt bacteria slide appeared to have more diplomatically and stereoscopically shapes in them. I did not see this with the blood smear, In the blood smear the shapes were more consistent with Cisco or staphylococci shapes.