2. Select gene of interest & insert into plasmid.
3. Plasmid taken up by bacterium.
4. Bacteria replicate
2 OPTIONS FROM HERE…
• Protein product harvested from bacteria.
• Create and harvest copies of a gene.
ex. a clone might refer to a culture of bacterial cells that contain a particular recombinant DNA plasmid, as in, “This is our clone of human insulin.”
A DNA sequence from a human, by itself, won’t do much if taken up by a bacterium. DNA molecules with exposed ends (i.e., noncircular) are degraded by bacterial enzymes.
Usually the goal is to produce many copies of the gene—either to harvest those DNA copies, or to produce (and harvest) large amounts of the gene’s protein product. I
These bacteria now produce hGH protein that is harvested and purified by pharmaceutical companies.
2. The gene for Bt toxin is isolated from the bacterium and inserted into the plasmid.
3.The plasmid is taken up by a bacterium.
4. The bacterium is used to introduce the Bt toxin gene into the chromosome of plant cells.
5. A single cell is chosen to produce a plant that now produces Bt toxin.
• The Bt toxin could negatively affect the taste of the food that is produced from these crops.
• The Bt toxin could result in the death of non-pest species of insects.
