Table 1: Color and Percent Transmittance between an amylase and starch solution with a pH of 6.0 tested in 3 minute intervals
Time
Order nowColor
Percent ( % ) Transmittance
Average Rate per minute
3
Dark Navy
6
Dark Navy
9
Dark Navy
12
Brown
0.2
0.0667
15
Dark Orange
8.2
2.6667
18
Orange
28.3
6.7
21
Orange
62.3
11.3333
24
Orange
67.4
1.7
27
Bright Orange
79.1
3.9
30
Bright Orange
85.3
2.0667
33
Bright Orange
87.7
0.8333
36
Bright Orange
90.2
-0.6333
39
Bright Orange
88.3
-0.6333
42
Bright Orange
83
-1.7667
45
Orange
74.4
-2.8667
48
Bright Orange
88.4
4.6667
51
Bright Orange
100
3.8667
Average rate=percent transmission at t 2 – per centum transmission at t1 / T 2 – T 1
Figure 1. Sum of visible radiation transmitted through a pH of 6.0 over clip in 3 minute intervals
Table 2: Color and Percent Transmittance between an amylase and starch solution with a pH of 7.0 tested in 3 minute intervals
Table 2
Time
Color
Percent ( % ) Transmittance
Average Rate per minute
3
Dark Navy
6
Dark Navy
9
Blue/Brown
0.1
0.0333
12
Dark Orange
3.5
1.1333
15
Orange
21.3
5.9333
18
Light Orange
67.1
15.2667
21
Light Orange
58.8
-2.7667
24
Light Orange
72.3
4.5
27
Bright Orange
76.3
1.3333
30
Bright Orange
81.1
1.6
33
Bright Orange
77.6
-1.1667
36
Bright Orange
85.5
2.6333
39
Bright Orange
73.4
-4.0333
42
Bright Orange
91.2
5.9333
45
Bright Orange
73.8
-5.8
48
Bright Orange
86.8
4.3333
51
Bright Orange
95.4
2.8667
54
Bright Orange
97.6
0.7333
57
Bright Orange
98.9
0.4333
60
Bright Orange
98.8
-0.0333
63
Bright Orange
91.8
-2.333
66
Bright Orange
100
2.7333
Average rate=percent transmission at t 2 – per centum transmission at t1 / T 2 – T 1
Figure 2. Sum of visible radiation transmitted through a pH of 7.0 over clip in 3 minute intervals
Table 3: Color and Percent Transmittance between an amylase and starch solution with a pH of 7.0 tested in 3 minute intervals
Table 3
Time
Color
Percent ( % ) Transmittance
Average Rate per minute
3
Dark Navy
6
Dark Navy
9
Blue/Brown
0.2
0.667
12
Dark Orange
10.1
3.3
15
Orange
33.8
7.9
18
Light Orange
48.6
4.9333
21
Light Orange
56.4
2.6
24
Light Orange
77.6
7.0667
27
Bright Orange
78.2
0.2
30
Bright Orange
83.7
1.8333
33
Bright Orange
80.1
-1.2
36
Bright Orange
88.5
2.8
39
Bright Orange
67.3
-7.0667
42
Bright Orange
82.2
4.9667
45
Bright Orange
78.2
-1.3333
48
Bright Orange
85.5
2.4333
51
Bright Orange
94.8
3.1
54
Bright Orange
82.4
-4.1333
57
Bright Orange
100
5.8667
Average rate=percent transmission at t 2 – per centum transmission at t1 / T 2 – T 1
Figure 3. Sum of visible radiation transmitted through a pH of 7.0 over clip in 3 minute intervals
Table 4: Color and Percent Transmittance between an amylase and starch solution with a pH of 8.0 tested in 3 minute intervals
Table 4
Time
Color
Percent ( % ) Transmittance
Average Rate per minute
3
Dark Navy
6
Dark Navy
9
Dark Brown
0.1
0.0333
12
Dark Brown
0.2
0.0333
15
Dark Orange
4.9
1.5667
18
Orange
19.4
4.8333
21
Orange
34.4
5
24
Orange
27.4
-2.3333
27
Orange
48.9
7.1667
30
Light Orange
58.1
3.0667
33
Light Orange
62.3
1.4
36
Light Orange
72.9
3.5333
39
Orange
64.6
-2.7667
42
Orange
78.7
4.7
45
Orange
73.5
-1.7333
48
Orange
74.1
0.2
51
Light Orange
80.8
2.2333
54
Bright Orange
87.3
2.1667
57
Bright Orange
88.5
0.4
60
Bright Orange
87.1
-0.4667
63
Bright Orange
87.8
0.2333
66
Bright Orange
90
0.7333
69
Bright Orange
90.5
0.1667
72
Bright Orange
82.4
-2.7
75
Bright Orange
78.9
-1.1667
78
Bright Orange
76.4
-0.8333
81
Bright Orange
93.1
5.5667
84
Bright Orange
94
0.3
87
Bright Orange
94.1
0.0333
90
Bright Orange
100
1.9667
Average rate=percent transmission at t 2 – per centum transmission at t1 / T 2 – T 1
Figure 4. Sum of visible radiation transmitted through a pH of 8.0 over clip in 3 minute intervals
Table 5: Color and Percent Transmittance between an amylase and starch solution with a pH of 8.0 tested in 3 minute intervals
Table 5
Time
Color
Percent ( % ) Transmittance
Average rate per minute
3
Dark Navy
6
Dark Navy
9
Dark Navy
12
Dark Navy
15
Dark Navy
18
Dark Navy
21
Dark Navy
24
Dark Navy
0.1
0.0333
27
Dark Brown
0.1
30
Dark Orange/Brown
0.1
33
Orange/Brown
0.4
0.1
36
Dark Orange
1.4
0.3333
39
Dark Orange
3.5
0.7
42
Dark Orange
11.1
2.5333
45
Dark Orange
10.5
-0.2
48
Dark Orange
16.2
1.9
51
Dark Orange
21.3
1.7
54
Dark Orange
26.4
1.7
57
Dark Orange
32.7
2.1
60
Dark Orange
33.5
0.2667
63
Orange
45.7
4.0667
66
Orange
46.3
0.2
69
Orange
42.1
-1.4
72
Orange
64.8
7.5667
75
Orange
52.5
-4.1
78
Orange
54.3
0.6
81
Orange
54.6
0.1
84
Orange
53.8
-0.2667
87
Orange
50
-1.2667
90
Orange
55.5
1.8333
93
Light Orange
65.7
3.4
96
Light Orange
62
-1.2333
Average rate=percent transmission at t 2 – per centum transmission at t1 / T 2 – T 1
Figure 5. Sum of visible radiation transmitted through a pH of 9.0 over clip in 3 minute intervals
Table 6. Entire Rate transmission per minute of different pH groups.
Changing pH
Rate transmission per minute
pH 6.0
1.961 ( % transmittance/min )
pH 7.0
1.515 ( % transmittance/min )
pH 7.0 ( control )
1.754 ( % transmittance/min )
pH 8
1.111 ( % transmittance/min )
pH 9
0.646 ( % transmittance/min )
Entire Rate = entire transmission / stoping clip
Figure 6. Percent Transmittance of an amylase amylum solution in 3 minute intervals with changing pH
Starch Hydrolysis Discussion
This experiment was conducted to demo if changing the pH in an environment will alter the rate of the reaction between amylum and amylase. The pH of the amylum amylase solution was the independent variable, and the sum of light being transmitted through the solution after adding I was the dependent variable. The tabular arraies and figures determine the reaction rate by plotting the per centum of visible radiation transmitted versus clip measured to acquire an equation. The void hypothesis of changing the pH of the enzyme solution will non impact the reaction between amylum and amylase is in wrong harmonizing to tabular arraies 1 -5. The changing pH groups in a solution had different rates harmonizing to table 6. The consequences showed that the first pH to make 100 % was the pH of 6.0. Harmonizing to table 1 it took 51 proceedingss for the pH 6.0 solution to make 100 % transmission. The solution of pH 7.0 took 57 proceedingss to make 100 % transmission harmonizing to table 3. Merely 6 proceedingss from the pH of 6.0. Harmonizing to table 2 the 2nd pH of 7.0 ( control ) showed 100 % transmission at 66 proceedingss. The solution of pH 8.0 took 90 proceedingss to make 100 % transmission harmonizing to table 4, most probably because it is more basic. The pH of 9.0 was either a dark naval forces or dark orange for most of the experiment. The solution of pH of 9.0 did non make 100 % transmission. Harmonizing to table 5 the pH of 9.0 solution took a sum of 96 proceedingss to make a transmission of merely 62 % .The pH of 9.0 solution ran out before it reached 100 % . Refering to the informations in figure 5 it took about 40 proceedingss earlier much visible radiation was transmitted for pH of 9.0. The solution of pH 9.0 had the slowest rate of the 5 changing pH solutions ; the rate was 0.646 harmonizing to table 6. The pH with the fastest rate was pH 6.0 with a rate of 1.961.
Mentioning the information from figure 6 the pH 0f 9.0 had the lowest per centum transmission. Not excessively far behind was the pH of 8.0. The solutions with a pH of 6.0, 7.0 and the control group were near in per centum transmission.
Decision
In decision the hypothesis for the amylum, amylase experiment was right. Yes changing the pH of the enzyme solution will impact the rate of the reaction between amylum and amylase. The 5 different pH groups, pH 6.0,7.0, 7.0 ( control ) 8.0 and 9.0 used in this experiment all had different reaction rates and different times when the per centum transmission reached 100 % .It was discovered that the information dropped a few times. This could hold been because the standardization was non completed plenty. This besides could hold been because cuvettes may hold had finger Markss and non decently wiped off. Another inquiry to be proposed would be, does changing the pH in different solutions change the reaction rate
between amylum and amylase?
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