Experiment #5 Separation and Purification of Organic Compounds l. Introduction Gumamela is an erect, much-branched, glabrous shrub, 1 to 4 meters high. Leaves are glossy green, ovate, acuminate, pointed, coarsely-toothed, 7 to 12 centimeters long, alternate, stipulate. Flowers are solitary, axillary, very large, about 10 centimeters long, and 12 centimeters in diameter. Outermost series of bracteoles are 6, lanceolate, green, and 8 millimeters long or less. Calyx is green, about 2 centimeters long, with ovate lobes. Petals are red, orange or rose-white, obovate, ntire, rounded tip, and imbricate.
Stamens form a long staminal tube enclosing the entire style of the pistil and protruding out of the corolla. Ovary is 5-celled, styles are 5, fused below. Fruits are capsules, loculicidally 5-valved, but rarely formed in cultivation. Results on flower extracts study suggest H. rosa-sinensis aids wound healing in the rat model. II. Materials Test Compounds Apparatus / Materials Reagents Red gumamela flowers Beakers 95% ethanol Pechay leaves Amber colored bottles 0. 1 N NaOH Separatory funnel 0. 1 N HCI Filter paper Petroleum ether
Vials Methanol Dessicator Distilled water Sodium sulfate A. Extraction of the Colored Substance from Gumamela Flowers Two petals of gumamela flowers were immersed in 25 mL of 95% ethanol in a beaker for one hour. The filtrate was collected, decanted and filtered in an amber colored bottle. The filtrate was tested for its indicator property by adding 1-2 drops of filtrate to 3 mL of 0. 1 N HCI solution to avail or test. Repeated using 3 mL of 0. 1 N NaOH solution. The results was recorded. B. Partial Solubility in Immiscible Solvents Extraction of Chlorophyll
The white stems of pechay leaves was removed and the green portion was washed thoroughly to remove dirt and soil adhering on the surface. The leaves were air dried. The green leaves was sliced finely and 10 grams of it was weighed. The leaves was immersed in a mixture of 45 mL petroleum ehter, and 15 mL of methanol. The mixture was set aside for one hour in a dark cool place. The liquid was decanted and filtered. The filtrate was placed in a separatory funnel and distilled water was added to dissolve the methanol. The green extract was collected in an evaporating dish. Avoid shaking vigorously.
The extract was dried over anhydrous sodium sulfate in a dessicator for 30 minutes. Its color and consistency was described. IV. Data and Results 0. 1 N Filtrate of gumamela petals Sample The filtrate of gumamela petals added 0. 1 N HCI turns into faint pink color The filtrate of gumamela petals added 0. 1 N NaOH turns into mint green color Before drying over an hydrous Sodium sulfate After drying Pechay leaves green extract Green liquid Green pigment V. Discussion of Results In table 1, after soaking the gumamela leaves in 95% ethanol,o. N HCI was added to the filtrate gumamela leaves and gives a faint pink color.
Whereas in 0. 1 N NaOH was added to the filtrate gumamela leaves gives a mint green color. anhydrous Sodium sulfate, it has a green liquid. After 30 minutes of drying the extracted pechay leaves, it has a green pigment which was the chlorophyll. VI. Application Study of ethanol flower extract on lipids and blood glucose of STZ-induced diabetic rats showed a lipid lowering effect (decrease cholesterol and serum triglycerides with increase in HDL) and diminution in blood glucose comparable to hat of glibenclamide but through a mechanism other than insulin release.
Study showed a hypolipidemic effect compared to the standard drug guggulipid. Histopath findings in rat liver supported the role of H rosa sinensis root extract in preventing cholesterol HFD-induced hepatic steatosis. Pak choi contains glucosinolates. These compounds have been reported to prevent cancer in small doses, but, like many substances, can be toxic to humans in large doses, particularly to people who are already seriously ill. In 2009, an elderly diabetic woman who had been consuming 1 o 1. kg of raw Pak choi per day, in an attempt to treat her diabetes, developed hypothyroidism, for reasons relating to her diabetes, resulting in myxedema coma. VI’. Conclusion The extraction of gumamela leaves in 95% ethanol gives a faint pink color, when 0. 1 N NaOH was added to the extracted gumamela leaves, the mixture gives a mint green color which indicated a presence of chlorophyll. Same in pechay leaves, after drying the fine sliced pechay leaves in an anhydrous Sodium sulfate, it gives a green pigment which was the chlorophyll itself.